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Part:BBa_K5230002:Design

Designed by: Minyi Yu   Group: iGEM24_UToronto   (2024-09-29)


Origin of replication for pColK_K235 PCR product


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4
    Illegal BsaI.rc site found at 1362


Design Notes

The BsaI sites and AAGG/TCAG overhangs were added via pColE1_RNAII_A_F and pColE1_RR_C_R primers.


Source

After aligning our machine learning generated origins of replication (OriV), the OriV for pColK_K235 was found to have the best alignment. The sequence for wild type pColK_K235 was found in the DoriC database.

References